Target-MPTPB(Mycobacterium Tuberculosis) H37Rv

Organism: MycoBacterium Tuberculosis H37Rv


Gene of Interest: MptpB Rv0153c

Background/Disease:

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NCBI Gene # or RefSeq#: NP_214667.1

EC# of Enzyme: 3.1.3.48

CAS Registry Number: 208861-82-9

CDS Gene Sequence:

ATGGCGGTTCGTGAACTGCCGGGTGCGTGGAACTTCCGCGACGTGGCTGACACCGCGACC
GCGCTGCGTCCGGGTCGTCTGTTCCGTAGCTCTGAACTGTCTCGTCTCGATGATGCCGGT
CGTGCCACCCTGCGCCGTCTGGGTATCACCGACGTTGCCGATCTCCGCTCTTCCCGTGAA
GTTGCTCGTCGTGGTCCAGGTCGTGTTCCAGATGGTATCGACGTTCACCTGCTGCCGTTT
CCTGATCTCGCGGACGATGACGCGGATGACTCTGCGCCTCACGAAACCGCATTCAAACGT
CTGCTGACCAACGACGGCTCTAATGGCGAATCTGGTGAATCTTCTCAGTCTATCAATGAT
GCAGCCACTCGTTACATGACCGACGAATATCGTCAATTCCCGACCCGTAATGGTGCGCAG
CGTGCCCTGCATCGTGTTGTAACCCTCCTGGCCGCTGGCCGTCCGGTTCTGACCCACTGC
TTTGCGGGTAAAGACCGTACCGGCTTCGTTGTTGCACTGGTTCTGGAAGCGGTTGGTCTG
GATCGTGACGTAATTGTGGCGGACTACCTCCGTTCTAACGACAGCGTTCCGCAACTGCGT
GCGCGTATCTCTGAAATGATCCAGCAGCGTTTCGACACCGAACTGGCACCGGAGGTTGTT
ACCTTCACCAAAGCGCGTCTGTCTGACGGCGTTCTCGGTGTTCGTGCGGAATACCTGGCA
GCGGCTCGTCAAACCATCGACGAAACTTACGGTTCTCTGGGTGGTTACCTGCGTGATGCG
GGCATCTCTCAGGCGACCGTTAACCGTATGCGTGGTGTTCTCCTGGGTTAA

Amino Acid Sequence (with stop codon, X, added at the end):

MptpB
Rv0153c
http://tuberculist.epfl.ch/quicksearch.php?gene+name=mptpb&submit=Search
>M. tuberculosis H37Rv | ptbB
MAVRELPGAWNFRDVADTATALRPGRLFRSSELSRLDDAGRATLRRLGITDVADLRSSREVARRGPGRVPDGIDVHLLPF
PDLADDDADDSAPHETAFKRLLTNDGSNGESGESSQSINDAATRYMTDEYRQFPTRNGAQRALHRVVTLLAAGRPVLTHC
FAGKDRTGFVVALVLEAVGLDRDVIVADYLRSNDSVPQLRARISEMIQQRFDTELAPEVVTFTKARLSDGVLGVRAEYLA
AARQTIDETYGSLGGYLRDAGISQATVNRMRGVLLGX

Length of protein in Amino Acids: 276

Molecular Weight of recombinant protein with MHHHHHHSSGVDLGTENLYFQSM N-terminal sequence: 32.71 kDa
Primer design results for pNIC-Bsa4 cloning:

Forward Primer:

TACTTCCAATCCATGGCGGTTCGTGAACTGCC


Reverse Primer:

TATCCACCTTTACTGTTAACCCAGGAGAACACCAC